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close this bookProtein-Energy Requirements of Developing Countries: Evaluation of New Data (UNU, 1981, 268 p.)
close this folderResearch papers: Protein requirements-adults, standard protocols
close this folderProtein requirements for young Colombian adults consuming local diets containing primarily animal or vegetable protein
View the document(introduction...)
View the documentObjectives
View the documentSummary of the main results
View the documentConclusions

Objectives

These studies were designed to determine the level of protein intake necessary to maintain nitrogen balance in a population living under the conditions of a developing tropical country. Two different local diets were examined.

Experimental Details

1. Subjects
For the first study, 11 healthy individuals consumed a predominantly animal protein diet (table IA). Eight healthy males took part in the second study of a predominantly vegetable diet (table IB).

2. Study Environment
Location: Universidad del Valle, Colombia. Climate: tropical.

3. Physical Activity
All subjects were students.

4. Diets
For the first study a menu largely made up of animal protein typical of the upper socio-economic class of Colombia was designed, as shown in table 2A. For the second study, the diet was patterned after that in the Cauca Valle region; 80 per cent of this diet is of vegetable origin (table 2B).

TABLE 1. Characteristics of the Subjects

 

1A. Animal Protein Diet

Subject Sex Age
(years)
Weight
(kg)
Height
(cm)
1 M 26 61 167
2 M 21 53 177
3 M 23 62.5 178
4 M 22 90.5 165
5 M 22 68 174
6 M 23 71.5 170
7 M 21 56 175
8 M 22 61 172
9 F 23 53 166
10 F 22 74 175
11 F 22 64 158
X   22.4 64.9 170
S.D.   1.37 10.9 0.061

1B. Vegetable Protein Diet

O.A.01 M 25 55.7 174
A.A.02 M 23 61.0 172
R.E.03 M 22 57.5 163
J.A.04 M 22 64.0 172
J.G.05 M 25 66.5 166
F.P.06 M 24 57.2 170
W.V. 07 M 23 57.7 174
J.D. 08 M 25 63.2 170
X   23.6 60.3 170.1
S. D.   1.3 3.8 3.89

TABLE 2A. Animal Protein Diet

Breakfast: cafe au fait
bread
margarine
marmalade
juice
Mid-morning: Guava candy, sweetened carbonated beverage
Lunch: soup
fried chicken
fried cassava
salad: carrots

cabbage
tomatoes
lettuce
onion
mayonnaise

banana with milk, cream
black coffee, sweetened carbonated beverage

Afternoon: baked plantain, sweetened carbonated beverage
Supper: fried meat
baked potato
salad
fruit
brown sugar water

Take-home snack: Guava candy and sweetened carbonated beverage

5. Experimental Design
For the animal protein study there were four periods; for the vegetable protein study there were seven. Each period consisted of a one-day protein-free diet, a five-day adaptation period, a five-day collection period, and a three-day break.

TABLE 2B. Predominantly Vegetable Protein Diet

Breakfast: orange juice with sugar
fried plantain and margarine
Mid-morning: guava candy and sweetened carbonated beverage
Lunch: sancocho:
typical soup made
of plantain, cassava,
potatoes, meat, oil,
tomatoes, onion,
and parsley
white rice
beans and potatoes
Afternoon: guava candy and sweetened carbonated beverage
Supper: bean soup
beans and potatoes
peaches
sweetened carbonated beverage

Take-home snack: guava candy and sweetened carbonated beverage

6. Nitrogen Balance
The nitrogen content of the diet, urine, and faeces was measured by the micro Kjeldahl technique. Miscellaneous losses were estimated at 5 mg/kg.