|Protein-Energy Requirements of Developing Countries: Evaluation of New Data (UNU, 1981, 268 p.)|
|Research papers: Protein requirements-adults, standard protocols|
|Protein requirements for young Colombian adults consuming local diets containing primarily animal or vegetable protein|
These studies were designed to determine the level of protein intake necessary to maintain nitrogen balance in a population living under the conditions of a developing tropical country. Two different local diets were examined.
For the first study, 11 healthy individuals consumed a predominantly animal protein diet (table IA). Eight healthy males took part in the second study of a predominantly vegetable diet (table IB).
2. Study Environment
Location: Universidad del Valle, Colombia. Climate: tropical.
3. Physical Activity
All subjects were students.
For the first study a menu largely made up of animal protein typical of the upper socio-economic class of Colombia was designed, as shown in table 2A. For the second study, the diet was patterned after that in the Cauca Valle region; 80 per cent of this diet is of vegetable origin (table 2B).
TABLE 1. Characteristics of the Subjects
1A. Animal Protein Diet
1B. Vegetable Protein Diet
TABLE 2A. Animal Protein Diet
|Mid-morning:||Guava candy, sweetened carbonated beverage|
banana with milk, cream
|Afternoon:||baked plantain, sweetened carbonated beverage|
|Supper:||fried meat |
brown sugar water
Take-home snack: Guava candy and sweetened carbonated beverage
5. Experimental Design
For the animal protein study there were four periods; for the vegetable protein study there were seven. Each period consisted of a one-day protein-free diet, a five-day adaptation period, a five-day collection period, and a three-day break.
TABLE 2B. Predominantly Vegetable Protein Diet
|Breakfast:||orange juice with sugar|
|fried plantain and margarine|
|Mid-morning:||guava candy and sweetened carbonated beverage|
|typical soup made|
|of plantain, cassava,|
|potatoes, meat, oil,|
|beans and potatoes|
|Afternoon:||guava candy and sweetened carbonated beverage|
|beans and potatoes|
|sweetened carbonated beverage|
Take-home snack: guava candy and sweetened carbonated beverage
6. Nitrogen Balance
The nitrogen content of the diet, urine, and faeces was measured by the micro Kjeldahl technique. Miscellaneous losses were estimated at 5 mg/kg.