![]() | Protein-Energy Requirements of Developing Countries: Evaluation of New Data (UNU, 1981, 268 p.) |
![]() | ![]() | Protein requirements-children |
![]() | ![]() | Protein requirements of pre-school children: milk and soybean protein isolate |
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Tables 3 and 4 summarize the results derived from the nitrogen balance studies. Table 5 shows that total plasma proteins decreased after nine days with an intake of 0.5 9 milk protein/kg/day and also after nine days with 0.75 9 soybean protein/kg/day.
Except for a decrease in urinary urea with the decrease in nitrogen intake, there were no consistent diet-related changes in the other biochemical indicators explored.
Rates of weight gain during the nine-day periods were slower with milk protein intakes of 0.5 9 milk protein/kg/day than with 1.00 or 1.25 g/kg/day (ANOVA: F3 34 = 3.473; least significant difference 13.1 g/day).
Although the mean weight gain was also lower with the lowest levels of soybean protein intakes, these did not differ from the higher levels because of the large inter individual variability.
TABLE 3. Data Derived from Nitrogen Balances: Milk
Child |
Digestibility |
Regression of apparent |
Nitrogen intake required to retain | ||
App. |
"True"a |
Y = a + bX |
r | ||
Descending design | |||||
A.A, |
81 |
96 |
- 68 + 0.690X |
0.991 |
133 |
C.R. |
76 |
91 |
- 74 + 0.922X |
0.970 |
106 |
I.G. |
88 |
104 |
- 16 + 0.652X |
0.994 |
61 |
H.A. |
78 |
92 |
- 24 + 0.546X |
0.972 |
88 |
W.G. |
76 |
91 |
- 49 + 0.765X |
0.988 |
95 |
D.B. |
78 |
92 |
- 42 + 0.718X |
0.992 |
92 |
Mean |
80 |
94 |
- 46 + 0.716X |
96 | |
S.D. |
5 |
5 |
23 .125 |
24 | |
Pooled data
(n=22) d |
- 51 + 0.766X |
0.914 |
98 | ||
Ascending design | |||||
W.M. |
83 |
97 |
- 34 + 0.725X |
0.995 |
80 |
M.V. |
75 |
91 |
- 72 + 0.642X |
0.997 |
150 |
A.Z. |
82 |
96 |
- 51 +0.816X |
0.999 |
92 |
I.T. |
78 |
93 |
- 16 + 0.552X |
0.958 |
72 |
Mean |
80 |
94 |
- 43 + 0.684X |
99 | |
S.D. |
4 |
3 |
24 .113 |
35 | |
Pooled data (n=16) |
- 48 + 0.718X |
0.836 |
100 | ||
Both designs | |||||
Mean |
80 |
94 |
- 45 + 0.703X |
97 | |
S.D. |
4 |
4 |
22 .115 |
27 | |
Pooled data (n=38) |
- 49 + 0.740X |
0.877 |
98 |
a "True" digestibility assumes obligatory faecal nitrogen loss of
20 mg/kg/day.
b Apparent balance = intakeurinary nitrogenfaecal
nitrogen.
c Allowing 15 mg N/kg/day for growth and 9 mg N/kg/day for
insensible losses.
d Using all data for the corresponding design; n =
number of determinations.
TABLE 4. Data Derived from Nitrogen Balances: Soybean Protein Isolate
Child |
Digestibility |
Regression of apparent Balance (Y) on intake (X) |
Nitrogen intake required to retain 24 mg N/kg/day c | ||
App. |
"True"a |
Y = a + bX |
r | ||
Descending design | |||||
A.A. | 81 | 96 | - 34 + 0.522X | 0.986 | 111 |
C.R. | 67 | 83 | - 52 + 0.558X | 0.985 | 136 |
I.G. | 82 | 95 | - 36 + 0.563X | 0.993 | 107 |
H.A. | 76 | 96 | - 27 + 0.473X | 0.992 | 108 |
W.G. | 73 | 89 | - 52 + 0.543X | 0.981 | 140 |
Mean | 76 | 91 | - 40 + 0.532X | 120 | |
S.D. | 6 | 5 | 11 .036 | 16 | |
Pooled data in
(n = 18) d | - 44 + 0.553 | 0.936 | 123 | ||
Ascending design | |||||
W.M. | 83 | 98 | - 26 + 0.545X | 0.998 | 92 |
W.M.A. | 80 | 95 | - 53 + 0.743X | 0.998 | 104 |
M.V. | 80 | 96 | - 68 + 0.654X | 0.965 | 141 |
A.Z. | 76 | 91 | - 63 + 0.698X | 0.975 | 125 |
I.T. | 77 | 92 | - 46 + 0.528X | 0.989 | 132 |
Mean | 79 | 94 | - 51 + 0.634X | 119 | |
S.D. | 3 | 3 | 16 .094 | 20 | |
Pooled data
(n = 18) | - 55 + 0.662X | 0.921 | 119 | ||
Both designs | |||||
Mean | 78 | 94 | - 46 + 0.583X | 120 | |
S.D. | 5 | 4 | 15 .086 | 17 | |
Pooled data
(n = 36) | - 50 + 0.610X | 0.920 | 121 |
a, b, c, d See footnotes in table 3.
TABLE 5. Plasma Protein Concentrations with Different Levels of Protein Intake (g/dl)
Protein intakes (g/kg/day) | ||||||||||
Descending design |
Ascending design | |||||||||
2.00 |
1.25 |
1.00 |
0.75 |
0.50 |
1.50* |
0.50 |
0.75 |
1.00 |
1.25 | |
Milk | ||||||||||
mean |
6.90 |
6 80 |
6.90 |
6.62 |
6.37 a |
6.90 |
6.50 b |
6.75 |
6.58 |
6.73 |
S.D. |
0.20 |
0.29 |
0.54 |
0.31 |
0.38 |
0.29 |
0.29 |
0.96 |
0.67 |
0.25 |
4/5 c |
3/6 |
4/6 |
6/6 |
4/4 |
2/4 |
3/4 |
2/3 | |||
Soybean protein | ||||||||||
mean |
6.94 |
6.76 |
6.64 |
6.28 b |
6.20 |
7.03 |
6.25 b |
6.22 |
6.30 |
6.78 |
S.D. |
0.23 |
0.43 |
0.21 |
0.16 |
0.57 |
0.36 |
0.44 |
0.43 |
0.64 |
0.53 |
3/5 |
315 |
5/5 |
2/4 |
4/4 |
3/5 |
3/5 |
2/5 |
a,b Differs from preceding protein intake (student's paired "t"
test): a: p< 0.01; b: p<0.05.
c Proportion of children with decreased
concentrations after nine days on the corresponding dietary protein intake.
* Basal period.
The RPV and RNR of the soybean protein isolate, compared with milk, were 83 per cent and 81 per cent, respectively.