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close this bookProtein-Energy Requirements of Developing Countries: Evaluation of New Data (UNU, 1981, 268 p.)
close this folderProtein requirements-children
close this folderProtein requirements of pre-school children: milk and soybean protein isolate
View the document(introduction...)
View the documentObjectives
View the documentExperimental details
View the documentSummary of main results
View the documentConclusions

Summary of main results

Tables 3 and 4 summarize the results derived from the nitrogen balance studies. Table 5 shows that total plasma proteins decreased after nine days with an intake of 0.5 9 milk protein/kg/day and also after nine days with 0.75 9 soybean protein/kg/day.

Except for a decrease in urinary urea with the decrease in nitrogen intake, there were no consistent diet-related changes in the other biochemical indicators explored.

Rates of weight gain during the nine-day periods were slower with milk protein intakes of 0.5 9 milk protein/kg/day than with 1.00 or 1.25 g/kg/day (ANOVA: F3 34 = 3.473; least significant difference 13.1 g/day).

Although the mean weight gain was also lower with the lowest levels of soybean protein intakes, these did not differ from the higher levels because of the large inter individual variability.

TABLE 3. Data Derived from Nitrogen Balances: Milk

 

Child

Digestibility
(% of intake)

Regression of apparent
Balance (Y) on intake (X)
(mg N/kg/day) b

Nitrogen intake required to retain
24 mg N/kg/day c

 

App.

"True"a

Y = a + bX

r

 
Descending design  
A.A,

81

96

- 68 + 0.690X

0.991

133

C.R.

76

91

- 74 + 0.922X

0.970

106

I.G.

88

104

- 16 + 0.652X

0.994

61

H.A.

78

92

- 24 + 0.546X

0.972

88

W.G.

76

91

- 49 + 0.765X

0.988

95

D.B.

78

92

- 42 + 0.718X

0.992

92

Mean

80

94

- 46 + 0.716X

 

96

S.D.

5

5

23 .125

 

24

Pooled data

(n=22) d

   

- 51 + 0.766X

0.914

98

Ascending design  
W.M.

83

97

- 34 + 0.725X

0.995

80

M.V.

75

91

- 72 + 0.642X

0.997

150

A.Z.

82

96

- 51 +0.816X

0.999

92

I.T.

78

93

- 16 + 0.552X

0.958

72

Mean

80

94

- 43 + 0.684X

 

99

S.D.

4

3

24 .113

 

35

Pooled data (n=16)    

- 48 + 0.718X

0.836

100

Both designs  
Mean

80

94

- 45 + 0.703X

 

97

S.D.

4

4

22 .115

 

27

Pooled data (n=38)    

- 49 + 0.740X

0.877

98

a "True" digestibility assumes obligatory faecal nitrogen loss of 20 mg/kg/day.
b Apparent balance = intake—urinary nitrogen—faecal nitrogen.
c Allowing 15 mg N/kg/day for growth and 9 mg N/kg/day for insensible losses.
d Using all data for the corresponding design; n = number of determinations.

TABLE 4. Data Derived from Nitrogen Balances: Soybean Protein Isolate

Child

Digestibility
(% of intake)

Regression of apparent Balance (Y) on intake (X)
(mg N/kg/day) b

Nitrogen intake required to retain 24 mg N/kg/day c

 

App.

"True"a

Y = a + bX

r

 
Descending design  
A.A. 81 96 - 34 + 0.522X 0.986 111
C.R. 67 83 - 52 + 0.558X 0.985 136
I.G. 82 95 - 36 + 0.563X 0.993 107
H.A. 76 96 - 27 + 0.473X 0.992 108
W.G. 73 89 - 52 + 0.543X 0.981 140
Mean 76 91 - 40 + 0.532X   120
S.D. 6 5 11 .036   16
Pooled data in

(n = 18) d

    - 44 + 0.553 0.936 123
Ascending design  
W.M. 83 98 - 26 + 0.545X 0.998 92
W.M.A. 80 95 - 53 + 0.743X 0.998 104
M.V. 80 96 - 68 + 0.654X 0.965 141
A.Z. 76 91 - 63 + 0.698X 0.975 125
I.T. 77 92 - 46 + 0.528X 0.989 132
Mean 79 94 - 51 + 0.634X   119
S.D. 3 3 16 .094   20
Pooled data

(n = 18)

    - 55 + 0.662X 0.921 119
Both designs  
Mean 78 94 - 46 + 0.583X   120
S.D. 5 4 15 .086   17
Pooled data

(n = 36)

    - 50 + 0.610X 0.920 121

a, b, c, d See footnotes in table 3.

TABLE 5. Plasma Protein Concentrations with Different Levels of Protein Intake (g/dl)

Protein intakes (g/kg/day)

 

Descending design

Ascending design

 

2.00

1.25

1.00

0.75

0.50

1.50*

0.50

0.75

1.00

1.25

Milk

mean

6.90

6 80

6.90

6.62

6.37 a

6.90

6.50 b

6.75

6.58

6.73

S.D.

0.20

0.29

0.54

0.31

0.38

0.29

0.29

0.96

0.67

0.25

   

4/5 c

3/6

4/6

6/6

 

4/4

2/4

3/4

2/3

Soybean protein

mean

6.94

6.76

6.64

6.28 b

6.20

7.03

6.25 b

6.22

6.30

6.78

S.D.

0.23

0.43

0.21

0.16

0.57

0.36

0.44

0.43

0.64

0.53

   

3/5

315

5/5

2/4

 

4/4

3/5

3/5

2/5

a,b Differs from preceding protein intake (student's paired "t" test): a: p< 0.01; b: p<0.05.
c Proportion of children with decreased concentrations after nine days on the corresponding dietary protein intake.
* Basal period.

 

The RPV and RNR of the soybean protein isolate, compared with milk, were 83 per cent and 81 per cent, respectively.